vector pact2 Search Results


vector pact2  (TaKaRa)
93
TaKaRa vector pact2
Vector Pact2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector pact2/product/TaKaRa
Average 93 stars, based on 1 article reviews
vector pact2 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
pact2 plasmid  (Promega)
90
Promega pact2 plasmid
(A) L929 wt330 cells, carrying an integrated wild type muIFN-β promoter fused to CAT reporter gene, were mock infected or infected by RVFV ZH or C13 or with NDV. Total cell extracts were prepared at 4, 6 and 8 h p.i. and CAT actvity was measured. (B) Non-confocal conventional fluorescence microscopy was used to analyze the nuclear distribution of NSs filaments in murine L929 cells infected by C13 or ZH. Presence of NSs filament detected using rabbit polyclonal anti-NSs antibody (green) or total DNA distribution revealed with Hoechst 33258 are shown respectively, in left and middle panels. Merged images are shown in right panels. Scale bars, 10 μm. (C) For yeast two-hybrid screening, AH109 yeast were co-transformed by <t>pACT2-SAP30</t> 1–152 that expressed Gal4 transactivating domain fused to the open reading frames corresponding to the 152 first aa of SAP30 and pGBKT7, pGBKT7-NSs ZH , pGBKT7-NSs C13 , pGBKT7-NSs TOS , or pGBKT7-NSs GER in which NSs from RVFV ZH or C13 or NSs proteins from Toscana (TOSV) and Germiston (GERV) bunyaviruses were fused to the Gal4 DNA-binding domain. The values of β galactosidase activity represent at least four independent experiments with SD bars. (D) Two-hybrid system using full length wild type NSs ZH or the deleted forms. The numbers indicate the amino acid position in the reference sequence. The sequence lacking amino acids 16–198 correspond to C13. (E) GST-NSs (left panel) or GST-SAP30 (right panel) was incubated with an extract from 293 cells transfected with the HA tagged-SAP30 expressing plasmid (left panel) or from ZH infected L929 cells (right panel). After extensive washing, the proteins bound to the beads were analysed by western blots using antibodies against HA (left panel) or NSs (right panel). The Coomassie blue staining showing that equivalent amounts of GST fusion proteins were loaded on the beads is not shown. (F) HEK 293 cells were transfected with either pCS2-Myc (lanes 1,2) or pCS2-Myc-SAP30 (lane 3) and either not infected (lane 1) or infected with ZH (lanes 2,3). Cell lysates were precipitated with anti-myc (9E10) antibody. Crude lysates (input) and the precipitated proteins (IP) were detected with anti-myc and anti-NSs antibodies.
Pact2 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pact2 plasmid/product/Promega
Average 90 stars, based on 1 article reviews
pact2 plasmid - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
two-hybrid vectors pact-2, ralb-pfbl23 and ralbd-pgad1318  (Institut Curie)
90
Institut Curie two-hybrid vectors pact-2, ralb-pfbl23 and ralbd-pgad1318
(A) L929 wt330 cells, carrying an integrated wild type muIFN-β promoter fused to CAT reporter gene, were mock infected or infected by RVFV ZH or C13 or with NDV. Total cell extracts were prepared at 4, 6 and 8 h p.i. and CAT actvity was measured. (B) Non-confocal conventional fluorescence microscopy was used to analyze the nuclear distribution of NSs filaments in murine L929 cells infected by C13 or ZH. Presence of NSs filament detected using rabbit polyclonal anti-NSs antibody (green) or total DNA distribution revealed with Hoechst 33258 are shown respectively, in left and middle panels. Merged images are shown in right panels. Scale bars, 10 μm. (C) For yeast two-hybrid screening, AH109 yeast were co-transformed by <t>pACT2-SAP30</t> 1–152 that expressed Gal4 transactivating domain fused to the open reading frames corresponding to the 152 first aa of SAP30 and pGBKT7, pGBKT7-NSs ZH , pGBKT7-NSs C13 , pGBKT7-NSs TOS , or pGBKT7-NSs GER in which NSs from RVFV ZH or C13 or NSs proteins from Toscana (TOSV) and Germiston (GERV) bunyaviruses were fused to the Gal4 DNA-binding domain. The values of β galactosidase activity represent at least four independent experiments with SD bars. (D) Two-hybrid system using full length wild type NSs ZH or the deleted forms. The numbers indicate the amino acid position in the reference sequence. The sequence lacking amino acids 16–198 correspond to C13. (E) GST-NSs (left panel) or GST-SAP30 (right panel) was incubated with an extract from 293 cells transfected with the HA tagged-SAP30 expressing plasmid (left panel) or from ZH infected L929 cells (right panel). After extensive washing, the proteins bound to the beads were analysed by western blots using antibodies against HA (left panel) or NSs (right panel). The Coomassie blue staining showing that equivalent amounts of GST fusion proteins were loaded on the beads is not shown. (F) HEK 293 cells were transfected with either pCS2-Myc (lanes 1,2) or pCS2-Myc-SAP30 (lane 3) and either not infected (lane 1) or infected with ZH (lanes 2,3). Cell lysates were precipitated with anti-myc (9E10) antibody. Crude lysates (input) and the precipitated proteins (IP) were detected with anti-myc and anti-NSs antibodies.
Two Hybrid Vectors Pact 2, Ralb Pfbl23 And Ralbd Pgad1318, supplied by Institut Curie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/two-hybrid vectors pact-2, ralb-pfbl23 and ralbd-pgad1318/product/Institut Curie
Average 90 stars, based on 1 article reviews
two-hybrid vectors pact-2, ralb-pfbl23 and ralbd-pgad1318 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


(A) L929 wt330 cells, carrying an integrated wild type muIFN-β promoter fused to CAT reporter gene, were mock infected or infected by RVFV ZH or C13 or with NDV. Total cell extracts were prepared at 4, 6 and 8 h p.i. and CAT actvity was measured. (B) Non-confocal conventional fluorescence microscopy was used to analyze the nuclear distribution of NSs filaments in murine L929 cells infected by C13 or ZH. Presence of NSs filament detected using rabbit polyclonal anti-NSs antibody (green) or total DNA distribution revealed with Hoechst 33258 are shown respectively, in left and middle panels. Merged images are shown in right panels. Scale bars, 10 μm. (C) For yeast two-hybrid screening, AH109 yeast were co-transformed by pACT2-SAP30 1–152 that expressed Gal4 transactivating domain fused to the open reading frames corresponding to the 152 first aa of SAP30 and pGBKT7, pGBKT7-NSs ZH , pGBKT7-NSs C13 , pGBKT7-NSs TOS , or pGBKT7-NSs GER in which NSs from RVFV ZH or C13 or NSs proteins from Toscana (TOSV) and Germiston (GERV) bunyaviruses were fused to the Gal4 DNA-binding domain. The values of β galactosidase activity represent at least four independent experiments with SD bars. (D) Two-hybrid system using full length wild type NSs ZH or the deleted forms. The numbers indicate the amino acid position in the reference sequence. The sequence lacking amino acids 16–198 correspond to C13. (E) GST-NSs (left panel) or GST-SAP30 (right panel) was incubated with an extract from 293 cells transfected with the HA tagged-SAP30 expressing plasmid (left panel) or from ZH infected L929 cells (right panel). After extensive washing, the proteins bound to the beads were analysed by western blots using antibodies against HA (left panel) or NSs (right panel). The Coomassie blue staining showing that equivalent amounts of GST fusion proteins were loaded on the beads is not shown. (F) HEK 293 cells were transfected with either pCS2-Myc (lanes 1,2) or pCS2-Myc-SAP30 (lane 3) and either not infected (lane 1) or infected with ZH (lanes 2,3). Cell lysates were precipitated with anti-myc (9E10) antibody. Crude lysates (input) and the precipitated proteins (IP) were detected with anti-myc and anti-NSs antibodies.

Journal: PLoS Pathogens

Article Title: A SAP30 Complex Inhibits IFN-β Expression in Rift Valley Fever Virus Infected Cells

doi: 10.1371/journal.ppat.0040013

Figure Lengend Snippet: (A) L929 wt330 cells, carrying an integrated wild type muIFN-β promoter fused to CAT reporter gene, were mock infected or infected by RVFV ZH or C13 or with NDV. Total cell extracts were prepared at 4, 6 and 8 h p.i. and CAT actvity was measured. (B) Non-confocal conventional fluorescence microscopy was used to analyze the nuclear distribution of NSs filaments in murine L929 cells infected by C13 or ZH. Presence of NSs filament detected using rabbit polyclonal anti-NSs antibody (green) or total DNA distribution revealed with Hoechst 33258 are shown respectively, in left and middle panels. Merged images are shown in right panels. Scale bars, 10 μm. (C) For yeast two-hybrid screening, AH109 yeast were co-transformed by pACT2-SAP30 1–152 that expressed Gal4 transactivating domain fused to the open reading frames corresponding to the 152 first aa of SAP30 and pGBKT7, pGBKT7-NSs ZH , pGBKT7-NSs C13 , pGBKT7-NSs TOS , or pGBKT7-NSs GER in which NSs from RVFV ZH or C13 or NSs proteins from Toscana (TOSV) and Germiston (GERV) bunyaviruses were fused to the Gal4 DNA-binding domain. The values of β galactosidase activity represent at least four independent experiments with SD bars. (D) Two-hybrid system using full length wild type NSs ZH or the deleted forms. The numbers indicate the amino acid position in the reference sequence. The sequence lacking amino acids 16–198 correspond to C13. (E) GST-NSs (left panel) or GST-SAP30 (right panel) was incubated with an extract from 293 cells transfected with the HA tagged-SAP30 expressing plasmid (left panel) or from ZH infected L929 cells (right panel). After extensive washing, the proteins bound to the beads were analysed by western blots using antibodies against HA (left panel) or NSs (right panel). The Coomassie blue staining showing that equivalent amounts of GST fusion proteins were loaded on the beads is not shown. (F) HEK 293 cells were transfected with either pCS2-Myc (lanes 1,2) or pCS2-Myc-SAP30 (lane 3) and either not infected (lane 1) or infected with ZH (lanes 2,3). Cell lysates were precipitated with anti-myc (9E10) antibody. Crude lysates (input) and the precipitated proteins (IP) were detected with anti-myc and anti-NSs antibodies.

Article Snippet: The plasmid pCi-HA-SAP30 was constructed by inserting the HA-tagged full length murine SAP30 cassette from the pACT2 plasmid into the pCi vector at the BglII site (Promega).

Techniques: Infection, Fluorescence, Microscopy, Two Hybrid Screening, Transformation Assay, Binding Assay, Activity Assay, Sequencing, Incubation, Transfection, Expressing, Plasmid Preparation, Western Blot, Staining